2019 CSCE Annual Conference - Laval (Greater Montreal)

2019 CSCE Annual Conference - Laval (Greater Montreal) Conference

Central Composite Design for Optimizing Derivatization of Metformin and Guanylurea in Water Samples Detected by Gas Chromatography - Mass Spectrometry

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Ms. Yunwen Tao, Memorial University of Newfoundland (Presenter)
Dr. Baiyu (Helen) Zhang, Memorial University
Dr. Bing Chen, Memorial University

Authors: Yunwen Tao, Baiyu Zhang, Bing Chen

Abstract: Emerging pollutants (EPs) are chemicals produced by human and industrial activities such as pharmaceuticals. Metformin, a widely used first-line oral drug for type 2 diabetes, is difficult to be metabolized inside the human body and thus becomes an emerging environmental contaminant with growing concerns. Guanylurea is metformin’s biotransformation product. To help better track the occurrence of the two compounds in the environments, the improvement of methods for metformin and guanylurea analysis is necessary. Derivatization of metformin and guanylurea is the key pre-treatment procedure before the analysis of metformin and guanylurea by gas chromatography-mass spectrometry (GC-MS). Central Composite design (CCD), a statistical design of experiments (DOE) methodology, was applied to identify the impact of factors affecting the derivatization reactions of metformin and guanylurea. The four factors included within the CCD modelling are temperature (70-90ºC), reacting time (40-70minutes), solvent (acetonitrile, 1,4-dioxane), and ratio (0.5-1.5:1) of reagent to target component. Buformin and N-methyl-bis(trifluoroacetamide) were used as the internal standard and derivatization reagent, respectively. The optimal conditions for enhancing the sensitization of metformin and guanylurea derivatization performance were obtained.